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a Heterologous ChAd/BNT/BNT or ChAd/ChAd/BNT vaccination increased total percentage of cytokine-secreting CD4 + ( a ) and CD8 + ( b ) T cells. We calculated the total number of cytokine secreting cells as the sum of IFN-γ + TNF-α − , IFN-γ + TNF-α + and IFN-γ − TNF-α + cells in the gates indicated in Extended Data Fig. 6. c, IFN-γ concentration in full blood supernatants <t>after</t> <t>stimulation</t> with SARS-CoV-2 S1 domain for 20–24 h measured by <t>IGRA</t> (Euroimmun). a – c Mixed effect analysis followed by Sidak’s multiple comparison test (within groups) and unpaired two-sided t test with Welch’s correction (between groups). The symbols depicted in grey had been published before , . Source data are provided as a Source Data file.
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a Heterologous ChAd/BNT/BNT or ChAd/ChAd/BNT vaccination increased total percentage of cytokine-secreting CD4 + ( a ) and CD8 + ( b ) T cells. We calculated the total number of cytokine secreting cells as the sum of IFN-γ + TNF-α − , IFN-γ + TNF-α + and IFN-γ − TNF-α + cells in the gates indicated in Extended Data Fig. 6. c, IFN-γ concentration in full blood supernatants <t>after</t> <t>stimulation</t> with SARS-CoV-2 S1 domain for 20–24 h measured by <t>IGRA</t> (Euroimmun). a – c Mixed effect analysis followed by Sidak’s multiple comparison test (within groups) and unpaired two-sided t test with Welch’s correction (between groups). The symbols depicted in grey had been published before , . Source data are provided as a Source Data file.
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a Heterologous ChAd/BNT/BNT or ChAd/ChAd/BNT vaccination increased total percentage of cytokine-secreting CD4 + ( a ) and CD8 + ( b ) T cells. We calculated the total number of cytokine secreting cells as the sum of IFN-γ + TNF-α − , IFN-γ + TNF-α + and IFN-γ − TNF-α + cells in the gates indicated in Extended Data Fig. 6. c, IFN-γ concentration in full blood supernatants <t>after</t> <t>stimulation</t> with SARS-CoV-2 S1 domain for 20–24 h measured by <t>IGRA</t> (Euroimmun). a – c Mixed effect analysis followed by Sidak’s multiple comparison test (within groups) and unpaired two-sided t test with Welch’s correction (between groups). The symbols depicted in grey had been published before , . Source data are provided as a Source Data file.
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a Heterologous ChAd/BNT/BNT or ChAd/ChAd/BNT vaccination increased total percentage of cytokine-secreting CD4 + ( a ) and CD8 + ( b ) T cells. We calculated the total number of cytokine secreting cells as the sum of IFN-γ + TNF-α − , IFN-γ + TNF-α + and IFN-γ − TNF-α + cells in the gates indicated in Extended Data Fig. 6. c, IFN-γ concentration in full blood supernatants <t>after</t> <t>stimulation</t> with SARS-CoV-2 S1 domain for 20–24 h measured by <t>IGRA</t> (Euroimmun). a – c Mixed effect analysis followed by Sidak’s multiple comparison test (within groups) and unpaired two-sided t test with Welch’s correction (between groups). The symbols depicted in grey had been published before , . Source data are provided as a Source Data file.
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a Heterologous ChAd/BNT/BNT or ChAd/ChAd/BNT vaccination increased total percentage of cytokine-secreting CD4 + ( a ) and CD8 + ( b ) T cells. We calculated the total number of cytokine secreting cells as the sum of IFN-γ + TNF-α − , IFN-γ + TNF-α + and IFN-γ − TNF-α + cells in the gates indicated in Extended Data Fig. 6. c, IFN-γ concentration in full blood supernatants <t>after</t> <t>stimulation</t> with SARS-CoV-2 S1 domain for 20–24 h measured by <t>IGRA</t> (Euroimmun). a – c Mixed effect analysis followed by Sidak’s multiple comparison test (within groups) and unpaired two-sided t test with Welch’s correction (between groups). The symbols depicted in grey had been published before , . Source data are provided as a Source Data file.
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Image Search Results


a Heterologous ChAd/BNT/BNT or ChAd/ChAd/BNT vaccination increased total percentage of cytokine-secreting CD4 + ( a ) and CD8 + ( b ) T cells. We calculated the total number of cytokine secreting cells as the sum of IFN-γ + TNF-α − , IFN-γ + TNF-α + and IFN-γ − TNF-α + cells in the gates indicated in Extended Data Fig. 6. c, IFN-γ concentration in full blood supernatants after stimulation with SARS-CoV-2 S1 domain for 20–24 h measured by IGRA (Euroimmun). a – c Mixed effect analysis followed by Sidak’s multiple comparison test (within groups) and unpaired two-sided t test with Welch’s correction (between groups). The symbols depicted in grey had been published before , . Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: BNT162b2-boosted immune responses six months after heterologous or homologous ChAdOx1nCoV-19/BNT162b2 vaccination against COVID-19

doi: 10.1038/s41467-022-32527-2

Figure Lengend Snippet: a Heterologous ChAd/BNT/BNT or ChAd/ChAd/BNT vaccination increased total percentage of cytokine-secreting CD4 + ( a ) and CD8 + ( b ) T cells. We calculated the total number of cytokine secreting cells as the sum of IFN-γ + TNF-α − , IFN-γ + TNF-α + and IFN-γ − TNF-α + cells in the gates indicated in Extended Data Fig. 6. c, IFN-γ concentration in full blood supernatants after stimulation with SARS-CoV-2 S1 domain for 20–24 h measured by IGRA (Euroimmun). a – c Mixed effect analysis followed by Sidak’s multiple comparison test (within groups) and unpaired two-sided t test with Welch’s correction (between groups). The symbols depicted in grey had been published before , . Source data are provided as a Source Data file.

Article Snippet: We calculated the total number of cytokine secreting cells as the sum of IFN-γ + TNF-α − , IFN-γ + TNF-α + and IFN-γ − TNF-α + cells in the gates indicated in Extended Data Fig. 6. c, IFN-γ concentration in full blood supernatants after stimulation with SARS-CoV-2 S1 domain for 20–24 h measured by IGRA (Euroimmun). a – c Mixed effect analysis followed by Sidak’s multiple comparison test (within groups) and unpaired two-sided t test with Welch’s correction (between groups).

Techniques: Concentration Assay, Comparison